Monday, November 1, 2010

CSI Acalanes "Who Done It!?"

         A life of crime is not as rewarding as it used to be. Using new technologies, it is easier to find the culprit based on DNA evidence. Restriction Fragment Length Polymorphism (RFLP) is the tool used when it come to DNA profiling. RFLP provides a unique banding pattern based on the restriction sties present in an individuals DNA sequence. In this lab we will compare different strands or patterns produced by restriction enzyme cleavage of DNA samples when separated on agarose gel. Agarose gel electrophoresis separates the DNA fragments by size since DNA fragments are negatively charged and they will be drawn towards the positive end. restriction enzymes are chemical scissors and it has recognition sequence on a segment of DNA of which it cuts the DNA at certain points. This evidence will show us who might be more likely have to committed the crime allowing us to send them to the brink, for a life of waiting and misery. however there are two main factors that affect the reliability of DNA fingerprinting. Population genetics and genetic statistics which skew or put in perspective the DNA evidence. Some populations show less variation in particular DNA segments than others so it seems more likely to be more than one person from that population.
         The propose of this lab is to discover who might be the possible culprit might be and to compare different strands of DNA using the RFLP technology. I hypothesize that we will easily find our killer or evil doer because the technology will be so advanced that it makes it obvious whose DNA it belongs to.
        Our results concluded that suspect number three or "Katie Records" was to blame for the murder of Trevor Stewart. We know that it was her because of the agarose technology showed that the DNA evidence found at he crime scene matched katies specific DNA strand. We believe this to be a crime of passion, and katies overwhelming love for trevor was too much for her to handle when he decided to walk out on her for another woman.
       Some possible sources of error might include, human error of contaminating the DNA before the electrophoresis. Another possible error is population sample, we may have wrongly accused someone who has a similar DNA sequence to someone in their relative population.  

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